By Elias Lianos, Elias A Lianos
ISBN-10: 0585237689
ISBN-13: 9780585237688
ISBN-10: 0896036677
ISBN-13: 9780896036673
In Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on specialists current state-of-the-art equipment for the research of eicosanoids, together with prostaglandins, thromboxanes, and leukotrienes. The without difficulty reproducible tools defined hereconcentrate on learning the law of expression and serve as of enzymes, really cyclooxygenase (and its isoforms), phospholipase A2, and lipoxygenases fascinated with the synthesis of confirmed eicosanoids. extra chapters are dedicated to the characterization and distribution of the thromboxane A2 receptor in tissues and the organic roles of novel eicosanoids. well timed and authoritative, the tools during this ebook may help their clients in exploring the pathobiology of irritation. Eicosanoid Protocols deals new and validated researchers strong, cutting-edge instruments to probe the law and serve as of eicosanoids.
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Extra info for Eicosanoid Protocols (Methods in Molecular Biology Vol 120)
Sample text
These switches can be programmed to be on, off, or to send a pulse. Note that the precolumn should initially be equilibrated with the loading solvent (15 or 30% methanol). The sequence is initiated by starting the autoinjector. Gr-cont, gradient controller; Autoini, autoinjector. 6. We have used two types of HPLC pumps as auxiliary pumps. The first is a Milton Roy/LDC minipump which was purchased a number of years ago and appears to be no longer available. The second is a Waters model 501 pump.
E. Methyl formate (Aldrich, 97% pure; redistilled) (see Note 3). f. 80% Ethanol in water. 2 Manual Technique for Precolumn Extraction of Eicosanoids Coupled to HPLC 1. Precolumn cartridge: Waters mBondapak C18 Guard-Pak inserts (Part number WAT088070) (see Note 4). 2. Precolumn cartridge holder: Waters Guard-Pak holder (part number WAT088141). For cartridges from other suppliers it will be necessary to use the appropriate cartridge holder. 3. , Valco Model C6W) (see Note 5). 4. Auxiliary pump (see Note 6).
However, this method is time consuming and can be complicated by the formation of emulsions, making it difficult to separate the two phases. To circumvent these problems we developed methods for the solid-phase extraction of eicosanoids on columns containing the hydrophobic stationary phase octadecylsilyl-silica (ODS-silica). This approach can be used either to obtain extracts containing a mixture of various eicosanoids (2,3) or can be From: Methods in Molecular Biology, vol. 120: Eicosanoid Protocols Edited by: E.
Eicosanoid Protocols (Methods in Molecular Biology Vol 120) by Elias Lianos, Elias A Lianos
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