By Mark A. Herbert, Derek W. Hood, E. Richard Moxon
ISBN-10: 0896039285
ISBN-13: 9780896039285
ISBN-10: 1592593216
ISBN-13: 9781592593217
In HaemophilusiInfluenzae Protocols, top examine scientists and infectious affliction experts aspect in a with no trouble reproducible layout the foremost molecular and immunological recommendations for exploring the pathogenicity of H. influenzae. defined with step-by-instructions to make sure strong and winning experimental effects, the options hide plasmid research, proteomics, genomics, DNA array know-how, gene expression, mutagenesis (transposon and nontransposon), and structural research. those equipment light up how the bacterium motives disorder, in addition to how top to advance novel vaccines and antibiotics opposed to the organism.
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Extra resources for Hemophilus Influenzae Protocols (Methods in Molecular Medicine)
Sample text
Influenzae lacks an O-antigen and thus consists of a simple set of monosaccharides (87). 1. Structure of LPS of H. influenzae The lipid A of H. influenzae consists of a β-(1,6)-1inked D-glucosamine disaccharide substituted by phosphate groups at C-1 of the reducing, and C-4 of the non-reducing D-glucosamine. The diglucosamine is further substituted by four molecules of (R)-3-hydroxytetradecanoic acid linked by amide linkages at the 2 and 2' positions and ester linkages at the 3 and 3' positions.
55, 513–520. Weiser, J. N. and Pan, N. (1998) Adaptation of Haemophilus influenzae to acquired and innate humoral immunity based on phase variation of lipopolysaccharide. Mol. Microbiol. 30, 767–775. Hood, D. , Deadman, M. , et al. (1996) Use of the complete genome sequence information of Haemophilus influenzae strain Rd to investigate lipopolysaccharide biosynthesis. Mol. Microbiol. 22, 951–965. Weiser, J. , and Chong, S. T. H. (1997) Decoration of lipopolysaccharide with phosphorylcholine: A phase-variable characteristic of Haemophilus influenzae.
4. Lipopolysaccharide Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria. A hydrophobic lipid moiety, lipid A, constitutes about 60% (w/w) of the LPS of H. influenzae ,while the remainder of this molecule consists of hydrophilic polysaccharide (86). Lipid A is embedded in the outer membrane, while the polysaccharide portion extends outward from the 36 Aubrey and Tang bacterial surface. As well as being an extremely important surface-exposed immunogen, LPS is essential for the integrity and functioning of the cell membrane.
Hemophilus Influenzae Protocols (Methods in Molecular Medicine) by Mark A. Herbert, Derek W. Hood, E. Richard Moxon
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