New PDF release: Structure and Assembly

By John E. Hesketh and Ian F. Pryme (Eds.)

ISBN-10: 1559386878

ISBN-13: 9781559386876

ISBN-10: 1559386886

ISBN-13: 9781559386883

ISBN-10: 1559386894

ISBN-13: 9781559386890

ISBN-10: 1559388455

ISBN-13: 9781559388450

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XL THE QUESTION OF REDUNDANCY IN ACTIN BINDING PROTEINS The huge number of ABPs in cells, and the high concentration at which some are present, may mean that an overprescription for actin-binding sites exists in cells, that is, there are more ABPs present than actin to bind them. , 1994). , transgelin and aldolase) in "physiological" salt concentrations are known to bind actin in cells (Pagliaro & 30 SUTHERLAND K. MACIVER Taylor, 1988), possibly through interactions with other ABPs. Another possible complication is that some ABPs are known to modulate the angular twist between subunits in the actin filament (Stokes & DeRosier, 1987), thereby altering (albeit subtly) the exposed surface of the actin filament, and concomitantly the type of ABP bound.

Sato, M. (1986). Characterization of alpha-actinin from Acantfiamoeba. Cell Mot. Cytoskel. 6, 649-661. G. (1994). Characterisation of the F-actin domains of villin: Classification of F-actin binding into two groups according to their binding sites on actin. FEBS Lett, (in press). C. B. (1991). Mbhl: A novel gelsolin/severin-related protein which binds actin in vitro and exhibits nuclear localization in vivo. EMBO J. 10, 757-766. D. (1993). Primary structure of Acanthamoeba actophorin. Biochemistry 32, 8525-8533.

This is not to say that all these interactions are explained totally by nonspecific charge interactions, since several lines of evidence point to the specificity of such interaction with at least some actin binding proteins. The interaction is especially convincing in the case of profilin, members of the gelsolin group, and a-actinin. , 1990). XL THE QUESTION OF REDUNDANCY IN ACTIN BINDING PROTEINS The huge number of ABPs in cells, and the high concentration at which some are present, may mean that an overprescription for actin-binding sites exists in cells, that is, there are more ABPs present than actin to bind them.

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Structure and Assembly by John E. Hesketh and Ian F. Pryme (Eds.)


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