Read e-book online The Epidemiology of Plant Diseases PDF

By B.M. Cooke

ISBN-10: 1402045794

ISBN-13: 9781402045790

ISBN-10: 1402045808

ISBN-13: 9781402045806

ISBN-10: 1402045816

ISBN-13: 9781402045813

Plant illness epidemiology is a dynamic technology that types an important a part of the research of plant pathology. This e-book brings jointly a workforce of 35 foreign specialists. each one bankruptcy bargains with a vital part of the topic and permits the reader to totally know how each one exerts its impression at the development of pathogen populations in plant populations over an outlined time scale. because the first variation of the textual content was once released in 1998, many new advancements have happened within the matters lined, rather molecular diagnostics, modelling, fungicide resistance and data know-how. the second one version of the booklet is a complete textual content on all points of plant sickness epidemiology that are supposed to function a useful reference paintings for these interested in this attention-grabbing technological know-how of crop crops.

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The flexibility and high throughput capabilities of DNA microarrays have tremendous potential for pathogen detection, identification, and genotyping in molecular diagnostic laboratories. DNA microarrays are typically composed of DNA probes that are bound to a solid substrate such as glass. Probes used for genotyping microarrays are either PCR products from cloned genes or oligonucloetide DNA. Each spot in the array lattice is composed of many identical probes that are complementary to the gene of interest.

Diseased roots traditionally take far longer to inspect than foliage because the plants have to be dug out or pulled up first, and even then in nearly every examination there is a delay if a thick covering of soil masks the symptoms (Dusunceli and Fox, 1992). Although methods based on nucleic acid can detect the presence of alien commensals within or on a host, none of the current methods that can be used to identify an unfamiliar pathogen is without problems. If the epidemiologist lacks appropriate training and experience, the traditional methods are ineffective without ready access to accurate disease descriptions that also identify the pathogens in some detail.

1999). , 1993) based on sequence variations present in 16S and 23S rDNA regions. , 1994) have also been used to design specific PCR assays. The introduction of real-time PCR in the last few years has improved and simplified methods for PCR-based quantification. Real-time PCR has many advantages over conventional PCR: it is faster and higher throughput is possible, post-reaction processing is not required as the amplified products are detected by a built-in fluorimeter thereby eliminating the risk of contamination, and it is more specific than conventional PCR if a specific probe is used in addition to the two specific primers.

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The Epidemiology of Plant Diseases by B.M. Cooke


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