By J.J. Thomson
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8. Effect of 2-deoxy-D-glucose, an inhibitor of glycosylation, on grapevine fanleaf virus (GFLV) content of shoot tip cultures of fanleafinfected V. rupestris Scheele. Proliferating shoot tip cultures were grown for 2 weeks in media containing various amounts of the drug. They were then homogenized in buffer and subjected to enzyme-linked immunosorbent assay (ELISA) for GFLV detection (Monette 1985). Data points are the means of triplicates. 5 20 40 60 80 100 DEOXYGLUCOSE CONC. (mg L- 1 ) from V.
Yamakawa et al. (1985) have recently succeeded in enhancing colony formation from protoplasts by using a feeder layer method. 1 Practical Applications Micropropagation of Select Genotypes The technology for grapevine micropropagation by shoot tip culture is well established and the resulting product appears to be genetically identical to the mother plant. The greatest obstacle to the widespread utilization of this technology is its relatively high cost. Chee et al. (1984) have estimated the variable production cost per vine at approximately 60 cents when plantlets were rooted in vitro, or 40 cents when the in vitro rooting phase was bypassed.
05). 3 mg/l or higher. At lower concentrations of NAA, numerous small roots were produced along with callus, but the rooted plantIets were difficult to transplant. 1 mg/l. Novak and Juvova (1982) also found that NAA 18 P. L. Monette promoted callus formation while lBA did not. 1 J,lM lBA. These authors reported that half-strength MS was superior to full strength MS, White's (1943) minerals, or White's minerals plus organics for rooting of axillary shoots. Leaf removal had a negative effect on rooting and best results were obtained with shoots 20- 25 mm long with three to five developed leaves.
Recollections and Reflections by J.J. Thomson
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