By Edward A Sydenham & C H V Sutherland: Harold Mattingly
ISBN-10: 0900696575
ISBN-13: 9780900696572
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Example text
These switches can be programmed to be on, off, or to send a pulse. Note that the precolumn should initially be equilibrated with the loading solvent (15 or 30% methanol). The sequence is initiated by starting the autoinjector. Gr-cont, gradient controller; Autoini, autoinjector. 6. We have used two types of HPLC pumps as auxiliary pumps. The first is a Milton Roy/LDC minipump which was purchased a number of years ago and appears to be no longer available. The second is a Waters model 501 pump.
E. Methyl formate (Aldrich, 97% pure; redistilled) (see Note 3). f. 80% Ethanol in water. 2 Manual Technique for Precolumn Extraction of Eicosanoids Coupled to HPLC 1. Precolumn cartridge: Waters mBondapak C18 Guard-Pak inserts (Part number WAT088070) (see Note 4). 2. Precolumn cartridge holder: Waters Guard-Pak holder (part number WAT088141). For cartridges from other suppliers it will be necessary to use the appropriate cartridge holder. 3. , Valco Model C6W) (see Note 5). 4. Auxiliary pump (see Note 6).
However, this method is time consuming and can be complicated by the formation of emulsions, making it difficult to separate the two phases. To circumvent these problems we developed methods for the solid-phase extraction of eicosanoids on columns containing the hydrophobic stationary phase octadecylsilyl-silica (ODS-silica). This approach can be used either to obtain extracts containing a mixture of various eicosanoids (2,3) or can be From: Methods in Molecular Biology, vol. 120: Eicosanoid Protocols Edited by: E.
Roman Imperial Coinage, Volume IV, Part II: Macrinus to Pupienus by Edward A Sydenham & C H V Sutherland: Harold Mattingly
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